Laminin beta 3 Antibody (CL3353)

Staining of rat rectum shows moderate positivity in basement membrane of glandular cells, as well as strong positivity in endothelial cells.

Staining of rat skin shows moderate to strong positivity in basement membrane basal epidermal cells.

Analysis in human skin and pancreas tissues. Corresponding Laminin beta 3 RNA-seq data are presented for the same tissues.

Staining of human pancreas shows no positivity in glandular cells as expected.

Staining of human skin shows moderate positivity in the basement membrane of the basal layer of epidermal cells.

Staining of A-431 cells showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Analysis in human lung tissue.

Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.

Staining of rat kidney shows strong positivity in basement membrane in cells in glomeruli.

Staining of human prostate shows moderate to strong positivity in basement membrane of glandular cells.

Staining of rat lung shows moderate to strong positivity in basement membrane of pneumocytes.

Staining of human fallopian tube shows moderate to strong positivity in basement membrane of glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL3353
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Laminin beta 3 Antibody (CL3353) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was developed using a recombinant protein derived from Q13751, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Laminin beta 3 Antibody (CL3353)

• BM600-125kDa
• Epiligrin subunit bata
• FLJ99565
• Kalinin B1 chain
• Kalinin subunit beta
• kalinin-140kDa
• LAM5
• Laminin 5
• Laminin B1k chain
• laminin S B3 chain
• laminin subunit beta-3
• laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD))
• laminin, beta 3
• Laminin-5 subunit beta
• LAMNB1
• Nicein subunit beta
• nicein-125kDa

Background

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMB3