L3MBTL2 Antibody - BSA Free

Western Blot: L3MBTL2 Antibody [NBP1-32621] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.

Chromatin Immunoprecipitation: L3MBTL2 Antibody [NBP1-32621] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of L3MBTL2 antibody The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.

Western Blot: L3MBTL2 Antibody [NBP1-32621] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of L3MBTL2 protein by 2. 5 ug of L3MBTL2 antibody 5% SDS-PAGE gel.

Western Blot: L3MBTL2 Antibody [NBP1-32621] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.

• Reactivity: Hu, Mu, Bv
• Applications: WB, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

L3MBTL2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit L3MBTL2 Antibody - BSA Free (NBP1-32621) is a polyclonal antibody validated for use in WB, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human L3MBTL2. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: L3MBTL2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation 1:500-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for L3MBTL2 Antibody - BSA Free

• dJ756G23.3
• DKFZP761I141
• H-l(3)mbt-l
• H-l(3)mbt-like protein 2
• l(3)mbt-like 2 (Drosophila)
• L(3)mbt-like protein 2
• L3MBT
• lethal(3)malignant brain tumor-like protein 2

Background

Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes,probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with achromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell intomitosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: L3MBTL2