L1TD1 Antibody (901258)

Western blot shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human L1TD1 Monoclonal Antibody (Catalog # MAB8317) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for L1TD1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

L1TD1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (upper panel) or differentiated for 4 days (lower panel), using Mouse Anti-Human L1TD1 Monoclonal Antibody (Catalog # MAB8317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 901258
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

L1TD1 Antibody (901258) Summary

• Immunogen: E. coli-derived recombinant human L1TD1
  Met1-Leu142
  Accession # Q5T7N2
• Specificity: Detects human L1TD1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: L1TD1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for L1TD1 Antibody (901258)

• ECAT11
• ES cell-associated protein 11
• FLJ10884
• L1TD1
• LINE-1 type transposase domain containing 1
• LINE-1 type transposase domain-containing protein 1
• MGC133253
• RP5-1155K23.3

Background

L1TD1 (LINE-1 type transposase domain-containing protein 1; also known as ES cell-associated protein 11 and FLJ10884) is an RNA binding protein with a reported molecular weight of approximately 100 kDa.  It is 865 amino acids (aa) in length and shares 43% aa identity with mouse L1TD1.  L1TD1 is a marker for undifferentiated pluripotent stem cells.  Knock down of its expression in these cells has been shown to decrease the expression of critical pluripotency factors such as Nanog and Oct-3/4.  L1TD1 function in stem cells is likely regulated by its interaction with factors such as LIN-28 and is suggested to regulate RNA processing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: L1TD1
• Uniprot:
  • Q5T7N2()