Kv7.4 Antibody

Western Blot: Kv7.4 Antibody [NB100-93448] - Analysis of Kv7.4 in Cerebellum lysate (35ug protein in RIPA buffer) using NB100-93448 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kv7.4 Antibody Summary

• Immunogen: Peptide with sequence C-DKGPSDAEVVDE corresponding to internal region according to NP_004691.2, NP_751895.1.
• Epitope: C-DKGPSDAEVVDE
• Specificity: This antibody is expected to recognise both reported isoforms (NP_004691.2; NP_751895.1); may cross-react in Mouse;
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KCNQ4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human brain (cerebellum) lysates (calculated MW of 71.2 kDa band according to NP_751895.1).
• Control:

  Kv7.4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kv7.4 Antibody

• DFNA2
• DFNA2A
• KCNQ4
• KQT-like 4
• Kv7.4
• potassium channel KQT-like 4
• Potassium channel subunit alpha KvLQT4
• potassium voltage-gated channel subfamily KQT member 4
• potassium voltage-gated channel, KQT-like subfamily, member 4
• Voltage-gated potassium channel subunit Kv7.4

Background

The protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. The encoded protein can form a homomultimeric potassium channel or possibly a heteromultimeric channel in association with the protein encoded by the KCNQ3 gene. Defects in this gene are a cause of nonsyndromic sensorineural deafness type 2 (DFNA2), an autosomal dominant form of progressive hearing loss. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kv7.4 Antibody (NB100-93448)(1)

Publication using NB100-93448

• Oshima K, Grimm CM, Corrales CE et al. Differential distribution of stem cells in the auditory and vestibular organs of the inner ear. J Assoc Res Otolaryngol 2007-03-01 [PMID: 17171473]

Bioinformatics

• Gene Symbol: KCNQ4
• Entrez:
  • 9132(Human)
• Uniprot:
  • NP_004691.2(Human)