Kv1.2 Antibody

Western Blot: Kv1.2 Antibody [NBP1-42818] - Membrane preparations from HEK-293 cells untransfected (lane 1), transfected with Kv1.2 (lanes 2 and 4) and transfected with Kv1.3 (lane 3) were subjected to 10% SDS-PAGE and were Western blotted with the Kv1.2 Antibody (NBP1-42818). Lane 4 was probed with NBP1-42818 that had been preincubated with the peptide used to generate NBP1-42818.

Product Discontinued

Kv1.2 Antibody Summary

• Immunogen: Synthetic peptide made to a portion of rat Kv1.2 (within residues 325-375). [Swiss-Prot# P63142]
• Localization: Membrane; Multi-pass membrane protein.
• Specificity: Does not cross-react with Kv1.3 and Kv3.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNA2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This Kv1.2 antibody is useful for Blocking/Neutralizing, Western blot and Immunoprecipitation. In WB a band can be seen at ~80 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, rabbit, bovine, porcine, canine, Xenopus and other vertebrates.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Kv1.2 Antibody

• EC 3.6.1.27
• EC 6.1.1
• HBK5
• HK4
• HUKIV
• KCNA2
• Kv1.2
• MGC50217
• MK2
• NGK1
• potassium channel
• potassium voltage-gated channel subfamily A member 2
• potassium voltage-gated channel, shaker-related subfamily, member 2
• RBK2
• Voltage-gated K(+) channel HuKIV
• Voltage-gated potassium channel HBK5
• voltage-gated potassium channel protein Kv1.2
• Voltage-gated potassium channel subunit Kv1.2

Background

Kv1.2 (Potassium voltage-gated channel subfamily A member 2) mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which K(+) ions may pass in accordance with their electrochemical gradient. Kv1.2 binds PDZ domains of DLG1, DLG2 and DLG4. The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kv1.2 Antibody (NBP1-42818)(1)

Publication using NBP1-42818

• Zhou BY, Ma W, Huang XY. Specific antibodies to the external vestibule of voltage-gated potassium channels block current. J Gen Physiol;111(4):555-63. 1998-04-01 [PMID: 9524138] (WB, Human)

FAQs for Kv1.2 Antibody (NBP1-42818). (Showing 1 - 1 of 1 FAQ).

1. Hi, I'm looking for an antibody that can bind to an extracellular region in the voltage-gated potassium channel Kv1.2. I found an antibodies on your website (eg cat. no. NBP1-42818) which have as an immunogen residues 325-375, and this region encompasses one of the extra-cellular loops. Is it possible to use one of these antibodies in a flow-cytometry experiment and have them bind to in-situ Kv1.2 channels in the membranes of intact cells?
   • NBP1-42818 has been validated to have blocking/neutralizing properties, which suggests that it binds to an extracellular region of the protein. We have not tested it in flow, and I can't guarantee that it will work. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.

Bioinformatics

• Gene Symbol: KCNA2
• Entrez:
  • 3737(Human)
  • 16490(Mouse)
  • 25468(Rat)
• Uniprot:
  • P63142(Rat)