Kv1.2 Antibody

Immunocytochemistry/Immunofluorescence: Kv1.2 Antibody [NBP1-42802] - Detection of Kv1.2 (Green) in 293 human embryonic kidney cells using NBP1-42802. Nuclei (Blue) are counterstained using Hoechst 33258.

Product Discontinued

Kv1.2 Antibody Summary

• Immunogen: Synthetic peptide made to a portion of rat Kv1.2 (within residues 50-100). [Swiss-Prot: P63142]
• Localization: Membrane; Multi-pass membrane protein
• Specificity: Does not cross-react with Kv3.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: In Western blot, a band can be seen at ~80 kDa.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kv1.2 Antibody

• EC 3.6.1.27
• EC 6.1.1
• HBK5
• HK4
• HUKIV
• KCNA2
• Kv1.2
• MGC50217
• MK2
• NGK1
• potassium channel
• potassium voltage-gated channel subfamily A member 2
• potassium voltage-gated channel, shaker-related subfamily, member 2
• RBK2
• Voltage-gated K(+) channel HuKIV
• Voltage-gated potassium channel HBK5
• voltage-gated potassium channel protein Kv1.2
• Voltage-gated potassium channel subunit Kv1.2

Background

Kv1.2 (KCNA2, Potassium voltage-gated channel subfamily A member 2) mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which K(+) ions may pass in accordance with their electrochemical gradient. Kv1.2 binds PDZ domains of DLG1, DLG2 and DLG4. The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kv1.2 Antibody (NBP1-42802)(3)

Publications using NBP1-42802

• Yang YM, Arsenault J, Bah A et al. Identification of a molecular locus for normalizing dysregulated GABA release from interneurons in the Fragile X brain. Mol. Psychiatry. 2018-09-17 [PMID: 30224722] (WB, Mouse)
• Joseph BK, Thakali KM, Pathan AR et al. Postsynaptic Density-95 (PSD95) Scaffolding of Shaker-type K+ Channelsin Smooth Muscle Cells Regulates the Diameter of Cerebral Arteries. J Physiol. 2011-09-12 [PMID: 21911612] (WB, ICC/IF, IP, Rat)
• Zhou BY, Ma W, Huang XY. Specific antibodies to the external vestibule of voltage-gated potassium channels block current. J Gen Physiol;111(4):555-63. 1998-04-01 [PMID: 9524138] (WB, Human)

Bioinformatics

• Gene Symbol: KCNA2
• Entrez:
  • 3737(Human)
  • 16490(Mouse)
  • 25468(Rat)
• Uniprot:
  • P63142(Rat)