Kv1 Antibody

Western Blot: Kv1 Antibody [NBP1-42788] - Membrane preparations from HEK-293 cells untransfected (lane 1) transfected with Kv1.2 (lanes 2 and 4) and transfected with Kv1.3 (lane 3) were subjected to 10% SDS-PAGE and were Western blotted with Kv1 Antibody (NBP1-42788). Lane 4 was probed with NBP1-42788 that had been preincubated with the peptide used to generate NBP1-42788.

Product Discontinued

Kv1 Antibody Summary

• Immunogen: Synthetic peptide made to rat Kv1.2 (within residues 50-100), which is identical in all members of the Kv1 family.
• Localization: Membrane; Multi-pass membrane protein
• Specificity: Reacts with all members of the Kv1 family. Does not cross-react with Kv3.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This Kv1 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at ~80 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, bovine, porcine, canine, Xenopus, Zebrafish and other vertebrates.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kv1 Antibody

• potassium channel
• shaker-related subfamily
• voltage-gated potassium channel

Background

Kv1 is a subfamily (also called the Shaker family) of voltage-gated potassium channels containing at least seven members. Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. The Kv1 subfamily may be important in the regulation of the fast repolarizing phase of action potentials in heart and thus may influence the duration of cardiac action potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kv1 Antibody (NBP1-42788)(1)

Publication using NBP1-42788

• Zhou BY, Ma W, Huang XY. Specific antibodies to the external vestibule of voltage-gated potassium channels block current. J Gen Physiol;111(4):555-63. 1998-04-01 [PMID: 9524138] (WB, Human)

Bioinformatics

• Gene Symbol: KCNA2
• Uniprot:
  • P63142(Rat)