KSR1 Antibody

Product Discontinued

KSR1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 350-402 of Human KSR.
• Specificity: KSR (R386) pAb detects endogenous levels of KSR protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KSR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of KSR (R386) pAb in extracts from NIH/3T3 cells.
  Immunohistochemistry (IHC) analyzes of KSR (R386)pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KSR1 Antibody

• kinase suppressor of ras 1
• kinase suppressor of ras
• KSRRSU2

Background

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 has intrinsic kinase activity towards serine/threonine residues and is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFbeta-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFbeta stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KSR1
• Entrez:
  • 8844(Human)
  • 16706(Mouse)