Kirrel3/NEPH2 Antibody (3A12) - Azide and BSA Free

Sandwich ELISA: Kirrel3/NEPH2 Antibody (3A12) [H00084623-M02] - Detection limit for recombinant GST tagged KIRREL3 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Kirrel3/NEPH2 Antibody (3A12) - Azide and BSA Free Summary

• Immunogen: KIRREL3 (NP_115920.1, 679 a.a. ~ 778 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHV
• Specificity: KIRREL3 - kin of IRRE like 3 (Drosophila) (3A12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIRREL3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Kirrel3/NEPH2 Antibody (3A12) - Azide and BSA Free

• KIAA1867MGC126824
• kin of IRRE like 3 (Drosophila)
• Kin of irregular chiasm-like protein 3
• kin of IRRE-like protein 3
• KIRRE
• Kirrel3
• KIRREPRO4502
• mKIRRE
• MRD4
• NEPH2
• NEPH2MGC126850
• nephrin-like 2
• Nephrin-like protein 2

Background

NEPH2 is a member of the nephrin-like protein family, which includes NEPH1 (KIRREL; MIM 607428) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kirrel3/NEPH2 Antibody (H00084623-M02)(1)

Publication using H00084623-M02

• Talkowski ME, Rosenfeld JA, Blumenthal I et al. Sequencing chromosomal abnormalities reveals neurodevelopmental loci that confer risk across diagnostic boundaries Cell 2012-04-27 [PMID: 22521361] (WB, Human)

Bioinformatics

• Gene Symbol: KIRREL3
• Entrez:
  • 84623(Human)
• Uniprot:
  • NP_115920()