KIR2DS4/CD158i Antibody (179315)

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 179315
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

KIR2DS4/CD158i Antibody (179315) Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DS4
  Accession # P43632
• Specificity: Detects KIR2DS4/CD158i in Western blots. In flow cytometry, this antibody stains cells transfected with KIR2DS4/CD158i. Does not stain cells transfected with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 3DL1, 3DL2, or 3DS1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DS4
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DS4/CD158i Antibody (179315)

• CD158 antigen-like family member I
• CD158i antigen
• CD158i
• cl-39
• killer cell immunoglobulin-like receptor 2DS4
• killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8
• killer inhibitory receptor 4-1-2
• KIR antigen 2DS4
• KIR2DS4
• KKA3KIR1D
• MGC120019
• MGC125315
• MGC125317
• MHC class I NK cell receptor
• natural killer cell inhibitory receptor
• Natural killer-associated transcript 8
• NKAT-8
• NKAT8KIR412
• P58 natural killer cell receptor clones CL-39/CL-17
• p58 NK receptor CL-39/CL-17

Background

KIR2DS4 is an activating immunoglobulin-like receptor expressed on NK cells and some T cells. It has been implicated in the recognition of some HLA-C alleles (1). A deletion variant encoding a single domain secreted isoform of KIR2DS4 has also been described (2).

1. Katz, G. et al. (2001) J. Immunol. 166:7260.
2. Maxwell, L. et al. (2002) Tissue Antigens 60:254.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DS4/CD158i Antibody (MAB1847)(2)

Publications using MAB1847

• MJW Sim, S Rajagopala, DM Altmann, RJ Boyton, PD Sun, EO Long Human NK cell receptor KIR2DS4 detects a conserved bacterial epitope presented by HLA-C Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 31138701] (Functional Assay, Mouse)
• Ashley Moffett Activating KIR2DS4 Is Expressed by Uterine NK Cells and Contributes to Successful Pregnancy J. Immunol., 2016;0(0):. 2016 [PMID: 27815424] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KIR2DS4
• Uniprot:
  • P43632()