KIR2DS4/CD158i Antibody (179315) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DS4/CD158i PE‑conjugated Monoclonal Antibody (Catalog # FAB1847P) and Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 179315
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

KIR2DS4/CD158i Antibody (179315) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DS4
  Accession # P43632
• Specificity: Detects KIR2DS4/CD158i in Western blots. In flow cytometry, this antibody stains cells transfected with KIR2DS4/CD158i. Does not stain cells transfected with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 3DL1, 3DL2, or 3DS1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DS4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DS4/CD158i Antibody (179315) [Phycoerythrin]

• CD158 antigen-like family member I
• CD158i antigen
• CD158i
• cl-39
• killer cell immunoglobulin-like receptor 2DS4
• killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8
• killer inhibitory receptor 4-1-2
• KIR antigen 2DS4
• KIR2DS4
• KKA3KIR1D
• MGC120019
• MGC125315
• MGC125317
• MHC class I NK cell receptor
• natural killer cell inhibitory receptor
• Natural killer-associated transcript 8
• NKAT-8
• NKAT8KIR412
• P58 natural killer cell receptor clones CL-39/CL-17
• p58 NK receptor CL-39/CL-17

Background

KIR2DS4 is an activating immunoglobulin-like receptor expressed on NK cells and some T cells. It has been implicated in the recognition of some HLA-C alleles (1). A deletion variant encoding a single domain secreted isoform of KIR2DS4 has also been described (2).

1. Katz, G. et al. (2001) J. Immunol. 166:7260.
2. Maxwell, L. et al. (2002) Tissue Antigens 60:254.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DS4/CD158i Antibody (FAB1847P)(1)

Publication using FAB1847P

• A Podhorzer, M Dirchwolf, A Machicote, S Belen, S Montal, S Paz, H Fainboim, LG Podestá, L Fainboim The Clinical Features of Patients with Chronic Hepatitis C Virus Infections Are Associated with Killer Cell Immunoglobulin-Like Receptor Genes and Their Expression on the Surface of Natural Killer Cells Front Immunol, 2018-01-05;8(0):1912. 2018-01-05 [PMID: 29354127] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KIR2DS4
• Uniprot:
  • P43632()