Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells.

The ED50 for this effect is 0.9-3.6 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived human KIR2DL5/CD158f protein
Human KIR2DL5
(His22 - His240)
Accession # NP_065396
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
His22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
KIR2DL5A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
50.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
66 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF

  • CD158f
  • CD158F1
  • killer cell immunoglobulin-like receptor 2DL5A
  • killer cell immunoglobulin-like receptor KIR2DL5A
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A
  • killer-cell Ig-like receptor
  • KIR2DL5
  • KIR2DL5.1
  • KIR2DL5.3
  • KIR2DL5A
  • KIR2DL5B

Background

KIR2DL5 (2DL5, designated CD158f) is a 60 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family (1, 2). KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1 - 3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (3). Like other inhibiting KIRs, KIR2DL5 has two ITIM domains within its long tail; however, one of these is atypical and may cause KIR2DL5 to be less inhibitory than most inhibiting KIRs (4). Gene duplication is thought to have resulted in individual genes coding for KIR2DL5 A and B proteins that vary by only 2 amino acids (5). Individuals may express either KIR2DL5A or KIR2DL5B, both proteins, or neither protein (5). Within the KIR family, KIR2DL5 and KIR2DL4 form a subfamily. The two share 79% amino acid sequence identity, have longer cytoplasmic tails than other KIR, and each has one D0 and one D2-type Ig-like domain (1). However, KIR2DL5 is only inhibitory, while KIR2DL4 has characteristics of both activating and inhibiting KIR.

  1. Vilches, C. et al. (2000) J. Immunol. 164:5797.
  2. Estefania, E. et al. (2007) J. Immunol. 178:4402.
  3. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  4. Yusa, S. et al. (2004) J. Immunol. 172:7385.
  5. Gomez-Lozano, N. et al. (2002) Immunogenetics 54:314.

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    Bioinformatics

    Gene Symbol KIR2DL5A
    Uniprot