KIF22 Antibody (5F3)

Western Blot: KIF22 Antibody (5F3) [NBP2-37552] - Western blot analysis using KID mouse mAb against MCF-7 (1) and Hela (2) cell lysate.

Immunohistochemistry: KIF22 Antibody (5F3) [NBP2-37552] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KID mouse mAb with DAB staining.

Western Blot: KIF22 Antibody (5F3) [NBP2-37552] - Western blot analysis using KID mAb against human KID (AA: 225-419) recombinant protein. (Expected MW is 47 kDa)

Immunohistochemistry: KIF22 Antibody (5F3) [NBP2-37552] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using KID mouse mAb with DAB staining.

ELISA: KIF22 Antibody (5F3) [NBP2-37552] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

KIF22 Antibody (5F3) Summary

• Immunogen: Purified recombinant fragment of human KID expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIF22
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for KIF22 Antibody (5F3)

• Kid
• KIDA-328A3.2
• kinesin family member 22
• kinesin-like 4
• Kinesin-like DNA-binding protein
• Kinesin-like protein 4
• kinesin-like protein KIF22
• KNSL4kinesin-like DNA-binding protein pseudogene
• OBP
• OBP-1
• OBP-2
• origin of plasmid DNA replication-binding protein
• oriP binding protein

Background

KIF22 is encoded by this gene is a member of kinesin-like protein family. This family of proteins are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF22