KIF20A Overexpression Lysate

Images

 
Western Blot: KIF20A Overexpression Lysate (Adult Normal) [NBL1-12291] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIF20A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KIF20A Overexpression Lysate Summary

Description
Transient overexpression lysate of kinesin family member 20A (KIF20A) The lysate was created in HEK293T cells, using Plasmid ID RC201100 and based on accession number NM_005733. The protein contains a C-MYC/DDK Tag.
Gene
KIF20A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
100.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KIF20A Overexpression Lysate

  • FLJ21151
  • GG10_2
  • kinesin family member 20A
  • kinesin-like protein KIF20A
  • mitotic kinesin-like protein 2
  • MKLP2
  • Rab6-interacting kinesin-like protein
  • RAB6KIFLkinesin-like (rabkinesin6)
  • rabkinesin-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-48291
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
NBP2-67446
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP1-33110
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NB100-294
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, MiAr, WB
NB100-563
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF8221
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
NBP1-91991
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-17874
Species: Hu
Applications: ELISA, IF, IHC, IHC-P, WB
NB500-181
Species: Dr, Hu, Ma, Mu, Pl, Po, Rt
Applications: IB, ICC/IF, IP, WB
NBP2-37471
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-57493
Species: Hu, Mu
Applications: IP, WB
NB100-294
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, MiAr, WB
NBP1-06979
Species: Hu
Applications: PEP-ELISA, WB
NBP2-97478
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-89033
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-88050
Species: Hu
Applications: IHC, IHC-P
NB500-201
Species: Ca, Fe, Gp, Ha, Hu, Mu, Rt
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NB500-201
Species: Ca, Fe, Gp, Ha, Hu, Mu, Rt
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NBL1-12291
Species: Hu
Applications: WB

Publications for KIF20A Lysate (NBL1-12291) (0)

There are no publications for KIF20A Lysate (NBL1-12291).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for KIF20A Lysate (NBL1-12291) (0)

There are no reviews for KIF20A Lysate (NBL1-12291). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KIF20A Lysate (NBL1-12291) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional KIF20A Products

Bioinformatics Tool for KIF20A Lysate (NBL1-12291)

Discover related pathways, diseases and genes to KIF20A Lysate (NBL1-12291). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for KIF20A Lysate (NBL1-12291)

Discover more about diseases related to KIF20A Lysate (NBL1-12291).
 

Pathways for KIF20A Lysate (NBL1-12291)

View related products by pathway.

Research Areas for KIF20A Lysate (NBL1-12291)

Find related products by research area.

Blogs on KIF20A

There are no specific blogs for KIF20A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KIF20A Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol KIF20A