KDM2B Antibody

Immunohistochemistry-Paraffin: KDM2B Antibody [NB100-1293] - Staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry: Goat Polyclonal KDM2B Antibody [NB100-1293] - (7µg/ml) staining of paraffin embedded Human Skin. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Control: Goat Polyclonal KDM2B Antibody [NB100-1293] - Negative Control showing staining of paraffin embedded Human Skin, with no primary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KDM2B Antibody Summary

• Immunogen: Peptide with sequence C-FGQVEEKLLQKLS corresponding to C-Terminus according to NP_115979.3.
• Specificity: This antibody is expected to recognize isoform a (NP_115979.3) only.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KDM2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection limit 1:32000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-7 ug/ml
• Application Notes: WB: An anonymous customer has reported bands at approx. 170+90 kDa band in lysates HeLa (calculated MW of 153 kDa band according to NP_115979.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KDM2B Antibody

• [Histone-H3]-lysine-36 demethylase 1B
• CXXC2
• CXXC2F-box protein FBL10
• CXXC-type zinc finger protein 2
• FBL10
• F-box and leucine-rich repeat protein 10JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein
• F-box protein FBL10
• FBXL10
• JHDM1B
• JHDM1BF-box/LRR-repeat protein 10
• JmjC domain-containing histone demethylation protein 1B
• jumonji C domain-containing histone demethylase 1B
• Jumonji domain-containing EMSY-interactor methyltransferase motif protein
• KDM2B
• lysine (K)-specific demethylase 2B
• lysine-specific demethylase 2B
• NDY1
• PCCX2
• PCCX2EC 1.14.11.27
• protein containing CXXC domain 2
• Protein JEMMA
• Protein-containing CXXC domain 2

Background

Fbxl10 (PCCX2) encodes a nuclear protein carrying a PHD finger, a CXXC domain, and an acidic region. The CXXC domain was found to be sufficient for binding to DNA. The acidic region exhibits a high transactivation ability, but the full-length protein was inactive due to regions which inhibited the acidic region, including the C-terminal region. PCCX1 is activated by proteolytic cleavage, which removes the C-terminal inhibitory region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KDM2B Antibody (NB100-1293)(1)

Publication using NB100-1293

• Fujino T, Hasegawa M, Shibata S et al. PCCX1, a novel DNA-binding protein with PHD finger and CXXC domain, is regulated by proteolysis. Biochem Biophys Res Commun 2000-05-10 [PMID: 10799292]

Bioinformatics

• Gene Symbol: KDM2B
• Entrez:
  • 84678(Human)
• Uniprot:
  • Q8NHM5(Human)