Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).
Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - KDEL in human primary fibroblasts. Antibody at 1:600 in PBST, 0.1% triton + 10% DS. O/N incubation at 4C. Donkey anti-mouse Alexa Fluor 488 at ...read more
Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469] - Analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - Analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12.
Use in immunoprecipitation reported in scientific literature (PMID: 8805786).
94 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 4.5 using NBP1-97469 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.2), 50% Glycerol
0.09% Sodium Azide
Protein G purified
Alternate Names for KDEL Antibody (10C3)
Endoplasmic reticulum resident protein 58
ER protein 58
KDEL (Lys-Asp-Glu-Leu) containing 1
KDEL motif-containing 1
KDEL motif-containing protein 1
The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Great ab for ICC-IF in human fibroblasts. Ab dilution 1:600 (higher dilution needed) in PBST (0.1% triton) + 10% DS. O/N incubation at 4ºC. Secondary Donkey anti-mouse Alexa fluor 488 1:500 in PBST (0.1% triton) + 10% DS 1h RT.
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