KDEL Antibody (10C3)

Images

 
Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).
Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - KDEL in human primary fibroblasts. Antibody at 1:600 in PBST, 0.1% triton + 10% DS. O/N incubation at 4C. Donkey anti-mouse Alexa Fluor 488 at ...read more
Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469] - Analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - Analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12.

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Ch, In, Ma, Pl, Pm, YeSpecies Glossary
Applications WB, EIA, EM, Flow, ICC/IF, IHC, IHC-P, IP
Clone
10C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

KDEL Antibody (10C3) Summary

Immunogen
Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
Marker
Endoplasmic Reticulum Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POGLUT2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Electron Microscopy 1:10 - 1:500
  • Enzyme Immunoassay
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 8805786).
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-97469 in the following applications:

Publications
Read Publications using
NBP1-97469 in the following applications:

  • 7 publications
  • IHC
    1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    7 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for KDEL Antibody (10C3)

  • Endoplasmic reticulum resident protein 58
  • EP58KDEL1
  • ER protein 58
  • ERp58
  • KDEL (Lys-Asp-Glu-Leu) containing 1
  • KDEL motif-containing 1
  • KDEL motif-containing protein 1
  • MGC5302

Background

The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-97469
Species: Hu, Mu, Rt, Av, Ch, In, Ma, Pl, Pm, Ye
Applications: WB, EIA, EM, Flow, ICC/IF, IHC, IHC-P, IP

Publications for KDEL Antibody (NBP1-97469)(25)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Plant, Primate, Yeast.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(7)
IHC
(1)
IHC-P
(1)
IP
(1)
WB
(7)
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Filter By Species
Human
(6)
Mouse
(4)
Rat
(1)
Chicken
(1)
Plant
(1)
Primate
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NBP1-97469 Applications Species
Wang G, Xu J, Zhao J et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice Nat Commun Jan 10 2020 [PMID: 31924786] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Chang MH, Brown MR, Liu Y et al. Cl- and H+ coupling properties and subcellular localizations of wildtype and disease-associated variants of the voltage-gated Cl-/H+ exchanger ClC-5 J. Biol. Chem. Dec 18 2019 [PMID: 31852738] (ICC/IF, Human) ICC/IF Human
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep Feb 5 2019 [PMID: 30723259] (IHC-P, Mouse) IHC-P Mouse
Hao Y, Chow AW, Yip WC et al. G protein-coupled estrogen receptor inhibits the P2Y receptor-mediated Ca(2+) signaling pathway in human airway epithelia. Pflugers Arch. Jun 6 2016 [PMID: 27271044] (ICC/IF, Human) ICC/IF Human
Kilaparty SP, Agarwal R, Singh P et al. Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response. Cell Stress Chaperones. 2016 Apr 02 [PMID: 27038811] (WB, Mouse)

Details:
KDEL monoclonal antibody was used for WB analysis of microsomal and cytosolic fractions of MC3T3-E1 cells which were incubated or not with 100 uM etoposide for 24 hours. The antibody detected 75 and 58 kDa bands - the 75 kDa protein was distributed in b
WB Mouse
S Tan et al. Nedd4 Family-interacting Protein 1 (Ndfip1) Is Required for the Exosomal Secretion of Nedd4 Family Proteins. J. Biol. Chem. 283, 32621. 2008 [PMID: 18819914] (ICC/IF, Human) ICC/IF Human
D Sitterlin et al. The Hepatitis B Virus Precore Protein Is Retrotransported from Endoplasmic Reticulum (ER) to Cytosol through the ER-associated Degradation Pathway. J. Biol. Chem. 283, 32352. 2008 [PMID: 18805786] (IP, Human) IP Human
Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked). [PMID: 18755873]
Y Saga et al. Pofut1 is required for the proper localization of the Notch receptor during mouse development. Mech. Dev. 125, 663. 2008 [PMID: 18547789] (ICC/IF, Mouse) ICC/IF Mouse
D Ito et al. Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases. Ann. Neurol. 61, 237. 2007 [PMID: 17387721] (WB, Human) WB Human
Show All 25 Publications.

Reviews for KDEL Antibody (NBP1-97469) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-97469:
Filter by Applications
WB
(1)
ICC
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot KDEL NBP1-97469
Enlarge
4
reviewed by:
Verified Customer
WB Human 10/31/2020
View

Summary

ApplicationWestern Blot
Sample TestedHuman primary fibroblast
SpeciesHuman
Lot08071825

Comments

CommentsPrimary antibody 1:1000 incubation O/N 4ºC in Milk 5% in PBST (0.1% Tween)
Immunocytochemistry KDEL NBP1-97469
Enlarge
5
reviewed by:
Verified Customer
ICC Human 02/25/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman primary fibroblast
SpeciesHuman
Lot08071825

Comments

CommentsGreat ab for ICC-IF in human fibroblasts. Ab dilution 1:600 (higher dilution needed) in PBST (0.1% triton) + 10% DS. O/N incubation at 4ºC. Secondary Donkey anti-mouse Alexa fluor 488 1:500 in PBST (0.1% triton) + 10% DS 1h RT.

Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

FAQs for KDEL Antibody (NBP1-97469) (0)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Verified Customer
10/31/2020
Application: WB
Species: Human

Verified Customer
02/25/2020
Application: ICC
Species: Human

Bioinformatics

Gene Symbol POGLUT2