KCNMB2 Overexpression Lysate (Native)

Images

 
Western Blot: KCNMB2 Overexpression Lysate (Adult Normal) [NBL1-12192] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KCNMB2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KCNMB2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC222841 and based on accession number NM_181361. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens potassium large conductance calcium-activated channel, subfamily M, beta member 2 (KCNMB2), transcript variant 1, mRNA.
Gene
KCNMB2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for KCNMB2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KCNMB2 Overexpression Lysate (Native)

  • BK channel subunit beta-2
  • BKbeta2
  • calcium-activated potassium channel subunit beta-2
  • Calcium-activated potassium channel, subfamily M subunit beta-2
  • Charybdotoxin receptor subunit beta-2
  • hbeta2
  • hbeta3
  • k(VCA)beta-2
  • large conductance calcium-activated potassium channel beta 2 subunit
  • large-conductance Ca2+-activated K+ channel beta2 subunit
  • Maxi K channel subunit beta-2
  • MaxiK channel beta 2 subunit
  • MGC22431
  • potassium large conductance calcium-activated channel, subfamily M, beta member2
  • slo-beta-2

Background

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which decreases the activation time of MaxiK alpha subunit currents. Two variants encoding the same protein have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for KCNMB2 Lysate (NBL1-12192) (0)

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Bioinformatics

Gene Symbol KCNMB2