KCNMB2 Antibody (S53-32) - BSA Free

Western Blot: KCNMB2 Antibody (S53-32) [NBP2-12917] - analysis of Human Cell line lysates showing detection of BK Beta2 Potassium Channel protein using Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody, Clone S53-32 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: KCNMB2 Antibody (S53-32) [NBP2-12917] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dottiness and dull.

Immunohistochemistry-Paraffin: KCNMB2 Antibody (S53-32) [NBP2-12917] - . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Lower epidermal cells positive, but quite dull.

Product Discontinued

KCNMB2 Antibody (S53-32) - BSA Free Summary

• Immunogen: Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2
• Localization: Membrane
• Specificity: Detects approx 27kDa. No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNMB2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:2000
• Application Notes: 1 ug/ml of BK Beta 2 Antibody was sufficient for detection of BK Channel beta2 in 10 ug of COS-1 cells transiently transfected with KBeta2 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KCNMB2 Antibody (S53-32) - BSA Free

• BK channel subunit beta-2
• BKbeta2
• calcium-activated potassium channel subunit beta-2
• Calcium-activated potassium channel, subfamily M subunit beta-2
• Charybdotoxin receptor subunit beta-2
• hbeta2
• hbeta3
• k(VCA)beta-2
• large conductance calcium-activated potassium channel beta 2 subunit
• large-conductance Ca2+-activated K+ channel beta2 subunit
• Maxi K channel subunit beta-2
• MaxiK channel beta 2 subunit
• MGC22431
• potassium large conductance calcium-activated channel, subfamily M, beta member2
• slo-beta-2

Background

1 ug/mL was sufficient for detection of BK Channel beta2 in 10ug of COS-1 cells transiently transfected with KBeta2 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNMB2