KAT2A/GCN5 Knockout 293T Cell Lysate

Product Discontinued

KAT2A/GCN5 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-17 bp deletion in exon1 and -2 bp deletion in exon1
• Gene: KAT2A

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for KAT2A/GCN5 Knockout 293T Cell Lysate

• EC 2.3.1.48
• GCN5 general control of amino-acid synthesis 5-like 2 (yeast)
• GCN5L2
• GCN5L2GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2
• GCN5MGC102791
• General control of amino acid synthesis protein 5-like 2
• General control of amino acid synthesis, yeast, homolog-like 2
• HGCN5
• Histone acetyltransferase GCN5
• histone acetyltransferase KAT2A
• hsGCN5
• K(lysine) acetyltransferase 2A
• KAT2A
• KAT2A/GCN5
• Lysine acetyltransferase 2A
• PCAF-B
• STAF97

Background

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: KAT2A