Goat anti-Human Kappa Light Chain Secondary Antibody

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Human Kappa Light Chain Secondary Antibody Summary

• Immunogen: Human Kappa Free Light Chain
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with kappa free light chains. No was detected against lambda light chain or other serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGKC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:30000 (Primary) 1:100-1:500 (for coating plates)
  • Immunocytochemistry/ Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:30000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kappa Light Chain Antibody (NB7480)(2)

Publications using NB7480

• Ishii Y, Tsukahara M, Wakamatsu K. A rapid method for simultaneous evaluation of free light chain content and aggregate content in culture media of Chinese hamster ovary cells expressing monoclonal antibodies for cell line screening. J Biosci Bioeng 2016-04-01 [PMID: 26467692]
• Rocchetti MT, Papale M, d'Apollo AM et al. Association of urinary laminin G-like 3 and free K light chains with disease activity and histological injury in IgA nephropathy Clin J Am Soc Nephrol 2013-04-20 [PMID: 23599406]

Bioinformatics

• Gene Symbol: IGKC