Goat anti-Human Kappa Light Chain Secondary Antibody

Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Human Kappa Light Chain Secondary Antibody Summary

• Immunogen: Human Kappa Light Chain
• Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No cross-reactivity was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGKC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200- 1:2000
  • Western Blot 1:1000 - 1:30000
• Application Notes: ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kappa Light Chain Antibody (NB7463)(9)

Publications using NB7463

• Luo G, Yogeshwar S, Lin L Et al. T cell reactivity to regulatory factor X4 in type 1 narcolepsy Sci Rep 2021-04-10 [PMID: 33837283]
  
  Details:
  Citation using the HRP version of this antibody.
• Smith B, Kiessling A, Lledo-Garcia R et al. Generation and characterization of a high affinity anti-human FcRn antibody, rozanolixizumab, and the effects of different molecular formats on the reduction of plasma IgG concentration MAbs 2018-08-22 [PMID: 30130439]
• Kiessling P, Lledo-Garcia R, Watanabe S et al. The FcRn inhibitor rozanolixizumab reduces human serum IgG concentration: A randomized phase 1 study Sci Transl Med 2017-11-03 [PMID: 29093180]
• Richards FM, Tape CJ, Jodrell DI et al. Anti-tumour effects of a specific anti-ADAM17 antibody in an ovarian cancer model in vivo. PLoS One. 2012-01-01 [PMID: 22792380]
  
  Details:
  Citation using the HRP form of this antibody.
• Wang G, Ujiie H, Shibaki A et al. Blockade of autoantibody-initiated tissue damage by using recombinant fab antibody fragments against pathogenic autoantigen. Am J Pathol. [PMID: 20042683]
  
  Details:
  Citation using the HRP form of this antibody.
• Suzuki N, Aoki D, Tamada Y et al. HMOCC-1, a human monoclonal antibody that inhibits adhesion of ovarian cancer cells to human mesothelial cells. Gynecol Oncol . [PMID: 15491748]
  
  Details:
  Citation using the HRP form of this antibody.
• Mukherjee J, Chios K, Fishwild D et al. Production and characterization of protective human antibodies against Shiga toxin 1. Infect Immun. [PMID: 12228326]
  
  Details:
  Citation using the HRP form of this antibody.
• Mukherjee J, Chios K, Fishwild D et al. Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infection. Infect Immun. [PMID: 11796590]
  
  Details:
  Citation using the HRP form of this antibody.
• Tomokiyo K, Teshima K, Nakatomi Y et al. Induction of acquired factor IX inhibitors in cynomolgus monkey (Macaca fascicularis): a new primate model of hemophilia B. Thromb Res. [PMID: 11369429]
  
  Details:
  Citation using the HRP form of this antibody.

Review for Kappa Light Chain Antibody (NB7463) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Natalie Erdmann ICC Human 04/08/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: JJN3
• Species: Human
• Lot: A6

Comments

• Comments: 1. Cells fixed in 4%PFA 10 minutes, wash PBS X 3
  2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3
  3. Block 10% donkey serum 1 hour
  4. 1/10,000 NB7463 O/N @4oC; wash PBS X 3
  5. 1/2000 donkey F(ab’)2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour @room temp (Jackson # 705-166-147); wash PBS X 3
  6. Olympus/BIOVIEW Cy3 filter 300ms exposure

Bioinformatics

• Gene Symbol: IGKC