Kappa Light Chain Antibody (A8B5) - BSA Free

Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - Surface staining of immunoglobulin kappa light chains in human peripheral blood with anti-kappa light chains (A8B5) Pacific BlueTM.

Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.

Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - Surface staining of immunoglobulin kappa light chains in human peripheral blood with anti-kappa light chains (A8B5) PE.

Separation of human Ig Kappa Light Chain positive lymphocytes (red-filled) from Ig Kappa Light Chain negative lymphocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (A8B5) purified antibody (concentration in sample 4 ug/ml, GAM APC).

• Reactivity: Hu, Gp(-), Gt(-), Ha(-), Rb(-), Sh(-)
• Applications: ELISA, Flow, IHC, CyTOF-ready
• Clone: A8B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Kappa Light Chain Antibody (A8B5) - BSA Free Summary

• Immunogen: The antibody A8B5 reacts withIGKC (22.5 kDa) of immunoglobulins.
• Specificity: The mouse monoclonal antibody 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGKC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:2000
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 22.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Kappa Light Chain Antibody (A8B5) - BSA Free

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGKC