Kallikrein 8/Neuropsin Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 8/Neuropsin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8
  Gln29-Gly260
  Accession # O60259
• Specificity: Detects human Kallikrein 8/Neuropsin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Neutralization 8 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 8/Neuropsin Antibody [Unconjugated]

• EC 3.4.21
• EC 3.4.21.118
• hK8
• HNP
• kallikrein 8 (neuropsin/ovasin)
• Kallikrein 8
• kallikrein-related peptidase 8
• KLK8 protein type 1
• KLK8 protein type 2
• KLK8
• neuropsin type 1
• neuropsin type 2
• Neuropsin
• NP
• NRPN
• Ovasin
• PRSS19
• PRSS19kallikrein-8
• Serine protease 19
• Serine protease TADG-14
• TADG14neuropsin
• Tumor-associated differentially expressed gene 14 protein

Background

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (residues 29 to 32) and the mature chain (residues 33 to 260). Isoform 2 is identical to isoform 1, except that a 45 amino acid segment is inserted in isoform 2 between residues 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8). This activity can be inhibited by rhSerpin A5, rhSerpin F2 and AEBSF (R&D Systems, Catalog # 1266-PI, 1470-PI and EI001, respectively). Recombinant human KLK8 produced by R&D Systems corresponds to isoform 1.

1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
2. Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
3. Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
4. Davies, B. et al. (2001) J. Neurosci. 21:6993.
5. Kishi, T. et al. (2003) Cancer Res. 63:2771.
6. Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
7. Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
8. Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 8/Neuropsin Antibody (AF2025)(2)

Publications using AF2025

• Tan , Frederic, Elowitz , Michael Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase. Proc Natl Acad Sci U S A, 2014-04-14;111(17):E1740-8. 2014-04-14 [PMID: 24733888] (Flow Cytometry, Mouse)
• Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma. Biol. Chem., 2007-11-01;388(11):1235-42. 2007-11-01 [PMID: 17976017] (IHC-P, Human)

Bioinformatics

• Gene Symbol: KLK8
• Uniprot:
  • O60259()