Kallikrein 6/Neurosin Antibody

Western Blot: Kallikrein 6/Neurosin Antibody [NBP1-51939] - Analysis of Kallikrein 6 in Mouse Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using NBP1-51939 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kallikrein 6/Neurosin Antibody Summary

• Immunogen: Peptide with sequence KEKPGVYTDVCTHIR corresponding to C-Terminus according to Uniprot Mouse NP_035307.1.
• Epitope: KEKPGVYTDVCTHIR
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in mouse liver lysates (calculated MW of 27.5 kDa band according to NP_035307.1). An additional band of unknown identity was also consistently observed at 48 kDa. Both bands were successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kallikrein 6/Neurosin Antibody

• Bssp
• EC 3.4.21
• EC 3.4.21.-
• hK6
• kallikrein 6 (neurosin, zyme)
• Kallikrein 6
• kallikrein-6
• kallikrein-related peptidase 6
• KLK6
• Klk7
• Neurosin
• Protease M
• protease, serine, 18
• PRSS18
• PRSS9
• PRSS9MGC9355
• Serine protease 18
• Serine protease 9
• SP59
• Zyme

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Kallikrein 6, or KLK6, shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. It degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. KLK6 may be involved in regulation of axon outgrowth following spinal cord injury. KLK6 has also been shown to promote cell proliferation, migration, and invasion and is upregulated in several types of cancers including colon and ovarian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 6/Neurosin Antibody (NBP1-51939)(1)

Publication using NBP1-51939

• Iwata A, Maruyama M, Akagi T et al. Alpha-synuclein degradation by serine protease neurosin: implication for pathogenesis of synucleinopathies. Hum Mol Genet 2003-10-15 [PMID: 12928483]

Bioinformatics

• Gene Symbol: KLK6
• Uniprot:
  • NP_035307.1(Mouse)