Kallikrein 6/Neurosin Antibody (265508)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 265508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 6/Neurosin Antibody (265508) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 6/Neurosin
  Leu22-Lys244
  Accession # Q92876
• Specificity: Detects human Kallikrein 6/Neurosin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Factor X, recombinant mouse HGFA, rhKLK3, 4, 5, 8, 10, 11, 15, rhThrombin, or rhuPA is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLK6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 6/Neurosin Antibody (265508)

• Bssp
• EC 3.4.21
• EC 3.4.21.-
• hK6
• kallikrein 6 (neurosin, zyme)
• Kallikrein 6
• kallikrein-6
• kallikrein-related peptidase 6
• KLK6
• Klk7
• Neurosin
• Protease M
• protease, serine, 18
• PRSS18
• PRSS9
• PRSS9MGC9355
• Serine protease 18
• Serine protease 9
• SP59
• Zyme

Background

Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members (1, 2). The genes of this family aligned in tandem on chromosome 19q13.4 represent the largest contiguous group of proteases within the human genome. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung (2, 3). Human Kallikrein 6 (KLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes (1‑3). Known protein substrates of KLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The level of KLK6 is reduced in brain extracts of Alzheimer patients and increased in serum of patients with ovarian cancer.

1. Emami, N. and E.P. Diamandis (2007) Clin Chim Acta 381:78.
2. Borgoño, C.A. and E.P. Diamandis (2004) Nat. Rev. Cancer, 4:876.
3. Paliouras, M. et al. (2007) Cancer Lett. 249:61.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 6/Neurosin Antibody (MAB2008)(4)

Publications using MAB2008

• Spencer B, Valera E, Rockenstein E, Trejo-Morales M, Adame A, Masliah E A brain-targeted, modified neurosin (kallikrein-6) reduces alpha-synuclein accumulation in a mouse model of multiple system atrophy. Mol Neurodegener, 2015-09-23;10(1):48. 2015-09-23 [PMID: 26394760] (Western Blot, Mouse)
• Wennstrom M, Surova Y, Hall S, Nilsson C, Minthon L, Bostrom F, Hansson O, Nielsen H Low CSF Levels of Both alpha-Synuclein and the alpha-Synuclein Cleaving Enzyme Neurosin in Patients with Synucleinopathy. PLoS ONE, 2013-01-08;8(1):e53250. 2013-01-08 [PMID: 23308173] (ELISA Development, Human)
• Spencer B, Michael S, Shen J, Kosberg K, Rockenstein E, Patrick C, Adame A, Masliah E Lentivirus mediated delivery of neurosin promotes clearance of wild-type alpha-synuclein and reduces the pathology in an alpha-synuclein model of LBD. Mol Ther, 2012-04-17;21(1):31-41. 2012-04-17 [PMID: 22508489] (Western Blot, Human)
• Bin L, Kim BE, Hall CF Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin. J. Invest. Dermatol., 2011-07-14;131(11):2213-22. 2011-07-14 [PMID: 21753780] (Neutralization, Human)

Bioinformatics

• Gene Symbol: KLK6
• Uniprot:
  • Q92876()