Kallikrein 2 Antibody

Western Blot: Kallikrein 2 Antibody [NBP1-68799] - Staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Kallikrein 2 Antibody [NBP1-68799] - Staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Discontinued

Kallikrein 2 Antibody Summary

• Immunogen: KLK2, internal region
• Epitope: C-KHQSLRPDEDSSHD
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005542.1; NP_001002231.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA 1:2000
  • Western Blot 1:1000

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kallikrein 2 Antibody

• EC 3.4.21
• EC 3.4.21.35
• glandular kallikrein 2
• Glandular kallikrein-1
• hGK-1
• hK2
• Kallikrein 2
• kallikrein 2, prostatic
• kallikrein-2
• kallikrein-related peptidase 2
• Klk1c2
• KLK2
• KLK2A2
• MGC12201
• Tissue kallikrein-2
• Ton

Background

Kallikreins (KLKs) are a subgroup of trypsin-like serine proteases and are implicated in carcinogenesis. Kallikrein 2 is most closely related to Kallikrein 3 (PSA), with 78% identity and with kallikrein 1, at 68% identity. Like Kallikrein 3, Kallikrein 2 is found in greatest abundance in the prostate, but at much lower levels than Kallikrein 3. In addition, Kallikrein 2 seems to be more labile than Kallikrein 1 or Kallikrein 3 and is often found in a cleaved form. Kallikrein 2 is inhibited by Protein C Inhibitor (PCI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLK2
• Entrez:
  • 3817(Human)