Kallikrein 11 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Kallikrein 11 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
  Ile54-Asn282
  Accession # Q9UBX7
• Specificity: Detects human Kallikrein 11 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 5, and rhKallikrein 9 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 11 Antibody [Alexa Fluor® 488]

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.4
• Hippostasin
• hK11
• Kallikrein 11
• kallikrein-related peptidase 11
• KLK11
• MGC33060
• PRSS20kallikrein-11
• Serine protease 20
• TLSPhippostasin
• Trypsin-like protease

Background

As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9UBX7()