JNK2 Antibody (SC05-85)

Western Blot: JNK2 Antibody (SC05-85) [NBP2-67553] - Analysis of JNK2 on SHG-44 cell lysates using anti-JNK2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: JNK2 Antibody (SC05-85) [NBP2-67553] - Staining JNK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JNK2 Antibody (SC05-85) [NBP2-67553] - Analysis of paraffin-embedded mouse brain tissue using anti-JNK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: JNK2 Antibody (SC05-85) [NBP2-67553] - Analysis of Hela cells with JNK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: JNK2 Antibody (SC05-85) [NBP2-67553] - Staining JNK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: JNK2 Antibody (SC05-85) [NBP2-67553] - Staining JNK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

JNK2 Antibody (SC05-85) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human JNK2 aa 341-394 / 424. (SwissProt: P45984 Human; SwissProt: Q9WTU6 Mouse; SwissProt: P49186 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for JNK2 Antibody (SC05-85)

• c-Jun kinase 2
• c-Jun N-terminal kinase 2
• EC 2.7.11
• EC 2.7.11.24
• JNK2
• JNK2A
• JNK2BETA
• JNK2JNK2ALPHA
• JNK-55
• Jun kinase
• MAP kinase 9
• MAPK 9
• MAPK9
• mitogen-activated protein kinase 9
• p54a
• p54aSAPK
• PRKM9JNK2B
• SAPK
• SAPK1 alpha
• Stress-activated protein kinase JNK2

Background

JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK9