JNK/JIP3 Antibody

Product Discontinued

JNK/JIP3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 615-664 of Human JIP-3.
• Specificity: JIP-3 (Q649) detects endogenous levels of JIP-3 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK8IP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of JIP3 (Q649) pAb in extracts from HeLa cells.
  Immunohistochemistry (IHC) analyzes of JIP3 (Q649) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 147 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for JNK/JIP3 Antibody

• C-jun-amino-terminal kinase interacting protein 3
• C-Jun-amino-terminal kinase-interacting protein 3
• DKFZp762N1113
• homolog of Drosophila Sunday driver 2
• JIP-3
• JIP3FLJ00027
• JNK MAP kinase scaffold protein 3
• JNK/SAPK-associated protein-1
• JNK/stress-activated protein kinase-associated protein 1
• JNK-interacting protein 3
• JSAP1
• KIAA1066SYD2
• mitogen-activated protein kinase 8 interacting protein 3
• Mitogen-activated protein kinase 8-interacting protein 3
• syd

Background

c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members, JNK2 and JNK3, have been identified. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP3, have been identified in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JNK/JIP3 Antibody (NBP1-00895)(3)

Publications using NBP1-00895

• Francis V, Alshafie W, Kumar R et al. The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamics The Journal of biological chemistry 2022-08-04 [PMID: 35933016] (ICC/IF, Rat)
  
  Details:
  IF-1:400
• Rafiq NM, Lyons LL, Gowrishankar S et al. JIP3 links lysosome transport to regulation of multiple components of the axonal cytoskeleton Communications biology 2022-01-10 [PMID: 35013510] (WB, Human)
• Ghosh Arundhati Sengupta, Wang Bei, Pozniak Christine D et al. DLK induces developmental neuronal degeneration via selective regulation of proapoptotic JNK activity. J Cell Biol 2011-01-01 [PMID: 21893599] (IF/IHC, IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: MAPK8IP3
• Entrez:
  • 23162(Human)
  • 30957(Mouse)
• Uniprot:
  • Q9UPT6(Human)
  • Q9ESN9(Mouse)