ISLR-2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ISLR-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ISLR-2
  Cys20-Leu590
  Accession # Q6UXK2
• Specificity: Detects human ISLR-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human ISLR-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ISLR2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ISLR-2 Antibody [Unconjugated]

• immunoglobulin superfamily containing leucine-rich repeat 2
• ISLR2
• ISLR-2
• KIAA1465
• leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein
• LINX
• UNQ1885/PRO4329

Background

ISLR-2 (Ig-superfamily containing LRR protein 2; also KIAA1465) is a 100‑110 kDa member of the LRR family of proteins. It is expressed on developing motor, sensory and interneurons. Mature human ISLR-2 is 727 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 571 aa extracellular domain (ECD) and a 135 aa cytoplasmic region. The ECD shows multiple LRRs (aa 50‑227) and one Ig-like domain (aa 233‑371). One alternate splice form may exist that shows a deletion of aa 160‑570. Over aa 20‑590, human ISLR-2 is 89% aa identical to both canine and mouse ISLR-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ISLR-2 Antibody (AF4526)(2)

Publications using AF4526

• Aojia Zhuang, Aobo Zhuang, Yijiao Chen, Zhaoyu Qin, Dexiang Zhu, Li Ren et al. Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes eLife 5/9/2023 [PMID: 37158593]
• S Abudureyim, N Asai, A Enomoto, L Weng, H Kobayashi, X Wang, C Chen, S Mii, M Takahashi Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure Sci Rep, 2018-05-08;8(1):7292. 2018-05-08 [PMID: 29739947] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ISLR2
• Uniprot:
  • Q6UXK2()