ISG15/UCRP Antibody

Images

 
Western Blot: ISG15/UCRP Antibody [NBP2-16995] - Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody. HRP-conjugated ...read more
Immunohistochemistry-Paraffin: ISG15 Antibody [NBP2-16995] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Western Blot: ISG15 Antibody [NBP2-16995] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE gel, diluted at 1:2000.
Western Blot: ISG15/UCRP Antibody [NBP2-16995] - Wild-type (WT) and Interferon gamma Receptor 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Interferon ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

ISG15/UCRP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ISG15. The exact sequence is proprietary.
Localization
Cytoplasm; Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ISG15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ISG15/UCRP Antibody

  • G1P2
  • G1P2interferon-induced 17-kDa/15-kDa protein
  • IFI15
  • IFI15hUCRP
  • interferon, alpha-inducible protein (clone IFI-15K)
  • Interferon-induced 15 kDa protein
  • Interferon-induced 17 kDa protein
  • interferon-stimulated protein, 15 kDa
  • IP17
  • ISG15 ubiquitin-like modifier
  • ISG15
  • Ubiquitin cross-reactive protein
  • ubiquitin-like protein ISG15
  • UCRP
  • UCRPIP17

Background

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ISG15
Entrez