ISG15 Activating Enzyme/UBE1L Antibody

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-D01P] - Analysis of UBA7 expression in human stomach.

Immunocytochemistry/Immunofluorescence: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-D01P] - Analysis of antibody to UBA7 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-D01P] - Analysis of UBA7 expression in transfected 293T cell line by UBA7 polyclonal antibody.Lane 1: UBA7 transfected lysate(111.70 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

ISG15 Activating Enzyme/UBE1L Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: UBA7 (NP_003326.2, 1 a.a. - 1012 a.a.) full-length human protein. MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL
• Specificity: Reacts with ubiquitin-like modifier activating enzyme 7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBA7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ISG15 Activating Enzyme/UBE1L Antibody

• D8
• D8UBA1, ubiquitin-activating enzyme E1 homolog B
• ISG15 Activating Enzyme/UBE1L
• UBA1B
• UBA7
• UBA7, ubiquitin-activating enzyme E1
• UBE1L
• UBE2
• UBE2MGC12713
• Ubiquitin-activating enzyme 7
• Ubiquitin-activating enzyme E1 homolog
• ubiquitin-activating enzyme E1-like
• ubiquitin-activating enzyme E1-related protein
• ubiquitin-activating enzyme-2
• ubiquitin-like modifier activating enzyme 7
• ubiquitin-like modifier-activating enzyme 7

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA7
• Entrez:
  • 7318(Human)
• Uniprot:
  • NP_003326.2(Human)