ISG15 Activating Enzyme/UBE1L Antibody (5B10)

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody (5B10) [NBP1-51649] - Western blot analysis using UBE1L mouse mAb against Raji (1) and THP-1 (2) cell lysate.

Product Discontinued

ISG15 Activating Enzyme/UBE1L Antibody (5B10) Summary

• Immunogen: Purified recombinant fragment of human UBE1L expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBA7
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500-1:2000
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for ISG15 Activating Enzyme/UBE1L Antibody (5B10)

• D8
• D8UBA1, ubiquitin-activating enzyme E1 homolog B
• ISG15 Activating Enzyme/UBE1L
• UBA1B
• UBA7
• UBA7, ubiquitin-activating enzyme E1
• UBE1L
• UBE2
• UBE2MGC12713
• Ubiquitin-activating enzyme 7
• Ubiquitin-activating enzyme E1 homolog
• ubiquitin-activating enzyme E1-like
• ubiquitin-activating enzyme E1-related protein
• ubiquitin-activating enzyme-2
• ubiquitin-like modifier activating enzyme 7
• ubiquitin-like modifier-activating enzyme 7

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA7