ISCU Antibody (OTI4F5) [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI4F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

ISCU Antibody (OTI4F5) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Full length human recombinant protein of human ISCU (NP_055116) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ISCU
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ISCU Antibody (OTI4F5) [Alexa Fluor™ Plus 594]

• 2310020H20Rik
• HML
• hnifU
• iron-sulfur cluster assembly enzyme ISCU, mitochondrial
• iron-sulfur cluster scaffold homolog (E. coli)
• IscU iron-sulfur cluster scaffold homolog (E. coli)
• IscU iron-sulfur cluster scaffold homolog
• IscU
• ISU2
• MGC74517
• NIFU
• NifU-like N-terminal domain containing
• NifU-like N-terminal domain-containing protein
• NifU-like protein
• NIFUN

Background

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ISCU