IRS4 Antibody (5B9) - Azide and BSA Free

Western Blot: IRS4 Antibody (5B9) [H00008471-M01] - IRS4 monoclonal antibody (M01), clone 5B9 Analysis of IRS4 expression in HeLa.

Sandwich ELISA: IRS4 Antibody (5B9) [H00008471-M01] - Detection limit for recombinant GST tagged IRS4 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 5B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IRS4 Antibody (5B9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IRS4 (NP_003595, 850 a.a. ~ 950 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTHEQREADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAE
• Specificity: IRS4 - insulin receptor substrate 4
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRS4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRS4 Antibody (5B9) - Azide and BSA Free

• insulin receptor substrate 4,160 kDa phosphotyrosine protein
• IRS-4pp160
• Phosphoprotein of 160 kDa
• PY160

Background

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation..

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRS4 Antibody (H00008471-M01)(1)

Publication using H00008471-M01

• Zhang XY, Guo H, Han B et al. Revealing A-Raf functions through its interactome Biochim. Biophys. Acta 2018-05-16 [PMID: 29777862] (WB, Human)

Bioinformatics

• Gene Symbol: IRS4
• Entrez:
  • 8471(Human)
• OMIM:
  • 603510(Human)
• Uniprot:
  • NP_003595(Human)