Recombinant Human IRGM His Protein

SDS-Page: IRGM Protein [NBP2-23131]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human IRGM His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 23-181 of Human IRGM
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKETLKIVSR TPVNITMAGD SGNGMSTFIS ALRNTGHEGK ASPPTELVKA TQRCASYFSS HFSNVVLWDL PGTGSATTTL ENYLMEMQFN RYDFIMVASA QFSMNHVMLA KTAEDMGKKF YIVWTKLDMD LSTGALPEVQ LLQIRENVLE NLQKERVCEY

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IRGM
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 20.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human IRGM His Protein

• IFI1
• immunity-related GTPase family M protein
• immunity-related GTPase family, M
• interferon-inducible protein 1
• IRGM1
• LPS-stimulated RAW 264.7 macrophage protein 47 homolog
• LRG47
• LRG-47
• LRG-47-like protein
• M1
• MGC149263
• MGC149264

Background

Immunity-related GTPase family M protein, also known as IRGM, is required for clearance of acute protozoan and bacterial infections. This protein functions in innate immune response probably through regulation of autophagy. It may regulate proinflammatory cytokine production and prevent endotoxemia upon infection and may also play a role in macrophages adhesion and motility. Recombinant human IRGM protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IRGM