Intersectin 2 Antibody

Western Blot: Intersectin 2 Antibody [NBP1-71833] - Detection of Human Intersectin 2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Intersectin 2 antibody NBP1-71833 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Intersectin 2 Antibody [NBP1-71833] - analysis of Intersectin 2 in mouse spleen lysates using anti-Intersectin 2 antibody. WT cells used as a positive control and ITSN2 knockdown sample used as a negative control. Image submitted by a verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Intersectin 2 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 550 and 600 of human Intersectin 2 using the numbering given in entry NP_006268.2 (GeneID 50618).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITSN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation
  • Knockdown Validated
• Application Notes: Intersectin 2 antibody validated for WB from a verified customer review. IP reactivity reported in scientific literature ( bioRxiv May 8 2019).

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Intersectin 2 Antibody

• intersectin 2
• KIAA1256SH3 domain-containing protein 1B
• PRO2015
• SH3 domain protein 1B
• SH3P18-like WASP associated protein
• SH3P18SH3D1Bintersectin-2
• SWA
• SWAPSH3P18-like WASP-associated protein

Background

This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. Alternatively spliced transcript variants have been found for this gene that encode three distinct isoforms. Additional variants have been found but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Intersectin 2 Antibody (NBP1-71833)(2)

Publications using NBP1-71833

• Gubar O, Croise P, Kropyvko S et al. The atypical Rho GTPase RhoU interacts with Intersectin-2 to regulate endosomal recycling pathways bioRxiv May 8 2019 (IP, Human)
• Burbage M, Gasparrini F, Aggarwal S et al. Tuning of in vivo cognate B-T cell interactions by Intersectin 2 is required for effective anti-viral B cell immunity Elife 2018 Jan 16 [PMID: 29337666] (WB, Mouse)

Review for Intersectin 2 Antibody (NBP1-71833) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: Mouse Spleen
• Species: Mouse
• Lot: A1

Blocking

• Blocking Details: 5% milk in TBS-T, 12h, room temperature.

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% milk in TBS-T or 5% BSA in TBS-T

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG HRP
• Secondary Manufacturer Cat#: 43R-1589-FIT
• Secondary Concentration: 1:10000

Details

• Detection Notes: SDS-PAGE, with Biorad mini-Protean TGX gels - 7.5%, 1min exposure, positive control - WT cells, negative - ITSN2 knock-down

Bioinformatics

• Gene Symbol: ITSN2
• Uniprot:
  • NP_006268.2(Human)