Intersectin 2 Antibody

Images

 
Western Blot: Intersectin 2 Antibody [NBP1-71833] - Detection of Human Intersectin 2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: ...read more
Western Blot: Intersectin 2 Antibody [NBP1-71833] - analysis of Intersectin 2 in mouse spleen lysates using anti-Intersectin 2 antibody. WT cells used as a positive control and ITSN2 knockdown sample used as a negative ...read more
Western Blot: Intersectin 2 Antibody [NBP1-71833] - Whole cell lysate (5, 15 and 50) from HeLa cells. Antibody used at 0.04 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

Order Details

Intersectin 2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 550 and 600 of human Intersectin 2 using the numbering given in entry NP_006268.2 (GeneID 50618).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITSN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Application Notes
This antibody works in Western Blot. Not recommended for IP.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-71833 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Intersectin 2 Antibody

  • intersectin 2
  • KIAA1256SH3 domain-containing protein 1B
  • PRO2015
  • SH3 domain protein 1B
  • SH3P18-like WASP associated protein
  • SH3P18SH3D1Bintersectin-2
  • SWA
  • SWAPSH3P18-like WASP-associated protein

Background

This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. Alternatively spliced transcript variants have been found for this gene that encode three distinct isoforms. Additional variants have been found but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Intersectin 2 Antibody (NBP1-71833) (0)

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Review for Intersectin 2 Antibody (NBP1-71833) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-71833:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Intersectin 2 NBP1-71833
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reviewed by:
Anonymous
WB Mouse 02/20/2015
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Summary

ApplicationWestern Blot
Sample TestedMouse Spleen
SpeciesMouse
LotA1

Blocking

Blocking Details5% milk in TBS-T, 12h, room temperature.

Primary Anitbody

Dilution Ratio1:1000 in 5% milk in TBS-T or 5% BSA in TBS-T

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP
Secondary Manufacturer Cat#43R-1589-FIT
Secondary Concentration1:10000

Details

Detection NotesSDS-PAGE, with Biorad mini-Protean TGX gels - 7.5%, 1min exposure, positive control - WT cells, negative - ITSN2 knock-down

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Intersectin 2 Antibody (NBP1-71833) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Intersectin 2 Antibody (NBP1-71833)

Discover related pathways, diseases and genes to Intersectin 2 Antibody (NBP1-71833). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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5
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4
1
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2
0
1
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Anonymous
02/20/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ITSN2
Uniprot