Integrin beta 6 Antibody [Biotin]

HT‑29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin beta 6 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF4155, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Integrin beta 6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin beta 6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Integrin beta 6 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Integrin beta 6
  Gly22-Asn707
  Accession # P18564
• Specificity: Detects human Integrin beta 6 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse Integrin beta 6 is observed, approximately 10% cross-reactivity with recombinant human (rh) Integrin beta 3 is observed, and less than 5% cross-reactivity with rhIntegrin beta 5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITGB6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 6 Antibody [Biotin]

• Integrin beta 6
• integrin beta-6
• integrin, beta 6
• ITGB6

Background

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB6
• Uniprot:
  • P18564()