ING3 Antibody

Immunohistochemistry-Paraffin: ING3 Antibody [NBP1-02460] - Staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/mL.

Product Discontinued

ING3 Antibody Summary

• Immunogen: Synthetic peptide corresponding to an internal region near aa 285-310 of human ING3.
• Specificity: aa 294-304 of Human ING3 protein (Inhibitor of growth family, member 3). This sequence only shows homology to isoform 1 for ING3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ING3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:40000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Western Blot 1:200 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Potassium Phosphate (pH 7.2), 0.15 M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ING3 Antibody

• FLJ20089
• ING2
• inhibitor of growth family, member 3
• inhibitor of growth protein 3
• MEAF4
• P47
• p47ING3Eaf4

Background

p47 ING3 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ING3
• Entrez:
  • 54556(Human)
  • 71777(Mouse)
• Uniprot:
  • Q9NXR8(Human)