ING2 Antibody

Western Blot: ING2 Antibody [NB100-1044] - Staining of HepG2 lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Primary incubated for 12 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ING2 Antibody Summary

• Immunogen: Peptide with sequence C-DKSTEKTKKDRRSR corresponding to C-Terminus according to NP_001555.1.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Goat
• Gene: ING2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 30 kDa band observed in hepatocarcinoma HEPG2 lysates (calculated MW of 32.8 kDa band according to NP_001555).
• Control:

  ING2 Overexpression Lysate

• Control Peptide:

  ING2 Peptide (NB100-1044PEP)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ING2 Antibody

• ING1Linhibitor of growth family, member 1-like
• ING1Lp
• Inhibitor of growth 1-like protein
• inhibitor of growth family, member 2
• inhibitor of growth protein 2
• p33ING2p32

Background

This protein is similar to ING1, a tumor suppressor protein that can bind TP53, inhibit cell growth, and induce apoptosis. This protein binds phosphoinositides (PtdInPs) at its plant homeodomain (PHD) domain and it acts as a PtdInPs receptor. PtdInPs binding regulates the ability of this protein to activate TP53 and TP53-dependent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ING2
• Entrez:
  • 3622(Human)
• Uniprot:
  • NP_001555(Human)