Importin4 Knockout 293T Cell Lysate

Product Discontinued

Importin4 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,10 bp deletion in exon 4 and 1 bp insertion in exon 4
• Gene: IPO4

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for Importin4 Knockout 293T Cell Lysate

• IMP4B
• importin 4
• importin-4

Background

IPO4, also known as Importin-4, consists of a 1,081 amino acid long isoform that is 119 kDa and a shorter 1,083 amino acid isoform that is 119 kDa, and is a nuclear transport receptor for localization signals in between the nucleus and the cytoplasm. This protein has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E. Disease research is currently being studied with relation to IPO4 and leukemia, hypoxia, and pancreatic cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IPO4