ILK Antibody (SC68-04)

Western Blot: ILK Antibody (SC68-04) [NBP2-67621] - Analysis of Integrin linked ILK on different lysates using anti-Integrin linked ILK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T

Immunocytochemistry/Immunofluorescence: ILK Antibody (SC68-04) [NBP2-67621] - Staining Integrin linked ILK in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ILK Antibody (SC68-04) [NBP2-67621] - Analysis of paraffin-embedded rat brain tissue using anti-Integrin linked ILK antibody. Counter stained with hematoxylin.

Flow Cytometry: ILK Antibody (SC68-04) [NBP2-67621] - Analysis of Jurkat cells with Integrin linked ILK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibo

Immunocytochemistry/Immunofluorescence: ILK Antibody (SC68-04) [NBP2-67621] - Staining Integrin linked ILK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

ILK Antibody (SC68-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human ILK aa 410-452. (SwissProt: Q13418 Human; SwissProt: O55222 Mouse; SwissProt: Q99J82 Rat)
• Localization: Cell junction, Cell membrane, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ILK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ILK Antibody (SC68-04)

• 59 kDa serine/threonine-protein kinase
• DKFZp686F1765
• EC 2.7.11.1
• ESTM24
• ILK
• ILK1
• ILK-1
• ILK2
• ILK-2
• integrin-linked kinase
• integrin-linked kinase-2
• integrin-linked protein kinase
• p59
• p59ILK

Background

Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILK