ILF2 Antibody (JU50-33)

Western Blot: ILF2 Antibody (JU50-33) [NBP2-75539] - Analysis of ILF2 on different cell lysates using anti-ILF2 antibody at 1/500 dilution.Positive control:Lane 1: Mouse testis tissue Lane 2: HL-60Lane 3: SH-SY-5Y

Immunohistochemistry-Paraffin: ILF2 Antibody (JU50-33) [NBP2-75539] - Analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. Counter stained with hematoxylin.

Flow Cytometry: ILF2 Antibody (JU50-33) [NBP2-75539] - Analysis of SH-SY-5Y cells with ILF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: ILF2 Antibody (JU50-33) [NBP2-75539] - Analysis of paraffin-embedded human tonsil tissue using anti-ILF2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ILF2 Antibody (JU50-33) [NBP2-75539] - Analysis of paraffin-embedded human liver cancer tissue using anti-ILF2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ILF2 Antibody (JU50-33) [NBP2-75539] - Analysis of paraffin-embedded human kidney tissue using anti-ILF2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ILF2 Antibody (JU50-33) [NBP2-75539] - Analysis of paraffin-embedded mouse testis tissue using anti-ILF2 antibody. Counter stained with hematoxylin.

Product Discontinued

ILF2 Antibody (JU50-33) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ILF2 aa 245-294 / 390. (SwissProt: Q12905 Human; SwissProt: Q9CXY6 Mouse; SwissProt: Q7TP98 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ILF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:20
  • Western Blot 1:500
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ILF2 Antibody (JU50-33)

• interleukin enhancer binding factor 2, 45kD
• interleukin enhancer binding factor 2, 45kDa
• interleukin enhancer-binding factor 2
• MGC8391
• NF45PRO3063
• Nuclear factor of activated T-cells 45 kDa
• nuclear factor of activated T-cells, 45-kDa

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILF2