ILF1 Antibody

Images

 
Western Blot: ILF1 Antibody [NB100-1285] - (0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ILF1 Antibody [NB100-1285] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more
Flow Cytometry: ILF1 Antibody [NB100-1285] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: ILF1 Antibody [NB100-1285] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ILF1 Antibody Summary

Immunogen
Peptide with sequence C-TPPAAVREKGVQN corresponding to C-Terminus according to NP_004505.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in human thymus lysate (calculated MW of 69.1 kDa band according to NP_004505.2. Approx 80kDa band observed in nuclear lysates of cell lines HEK293, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004505.2.
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-1285.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ILF1 Antibody

  • Cellular transcription factor ILF-1
  • forkhead box K2
  • forkhead box protein K2
  • FOXK1
  • ILF-1
  • ILF1ILF
  • interleukin enhancer binding factor 1
  • Interleukin enhancer-binding factor 1

Background

The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILF1 Antibody (NB100-1285)(2)

We have publications tested in 1 confirmed species: Human.


Filter By Application
All Applications
Filter By Species
Human
(1)
All Species

Reviews for ILF1 Antibody (NB100-1285) (0)

There are no reviews for ILF1 Antibody (NB100-1285). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ILF1 Antibody (NB100-1285) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ILF1 Products

Research Areas for ILF1 Antibody (NB100-1285)

Find related products by research area.

Blogs on ILF1

There are no specific blogs for ILF1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ILF1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol FOXK2
Entrez
Uniprot