Recombinant Mouse IL-36 gamma/IL-1F9 Animal-Free Protein

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SDS-Page: Recombinant Mouse IL-36 gamma/IL-1F9 Animal-Free Protein [NBP2-76223] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE
Format
Animal Free
Concentration
LYOPH

Order Details

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Recombinant Mouse IL-36 gamma/IL-1F9 Animal-Free Protein Summary

Description
An un-tagged recombinant protein corresponding to Mouse IL-36 gamma/IL-1F9

Source: E. coli

Amino Acid Sequence: MGRETPDFGE VFDLDQQVWI FRNQALVTVP RSHRVTPVSV TILPCKYPES LEQDKGIAIY LGIQNPDKCL FCKEVNGHPT LLLKEEKILD LYHHPEPMKP FLFYHTRTGG TSTFESVAFP GHYIASSKTG NPIFLTSKKG EYYNINFNLD IKS

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
Specificity
IL-36 gamma/IL-1F9 is a monomer, 17.5 kDa (153 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL36G
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile 10 mM sterile HCI at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse IL-36 gamma/IL-1F9 Animal-Free Protein

  • IL-1 epsilon
  • IL-1 H1
  • IL-1 Related Protein 2
  • IL-1(EPSILON)
  • IL1E
  • IL-1-epsilon
  • IL1F9
  • IL-1F9
  • IL1H1
  • IL-1H1
  • IL-1-Related Protein 2
  • IL1RP2
  • IL-1rp2
  • IL36 gamma
  • IL-36 gamma
  • IL36G
  • interleukin 1 family, member 9
  • interleukin 1-related protein 2
  • Interleukin 36, Gamma
  • Interleukin-1 epsilon
  • interleukin-1 family member 9
  • Interleukin-1 homolog 1
  • Interleukin-36 Gamma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL36G