IL-36 gamma/IL-1F9 Antibody Pair

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell Culture Supernatant, Serum
• Standard Curve Range: 3.9 - 250 pg/ml
• Sensitivity: 1 pg/ml

IL-36 gamma/IL-1F9 Antibody Pair Summary

• Description: Sandwich ELISA for the quantitative determination of Human IL-36 gamma/IL-1F9
• Standard Curve Range: 3.9 - 250 pg/ml
• Sensitivity: 1 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: IL36G

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA
• Application Notes: 1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (mouse monoclonal antibody) to 2 ug/ml in PBS without carrier protein and use it fresh.
  2. Dilute the desired amount of Detection Antibody (DET) (0.1 mg/ml) (mouse monoclonal antibody) to 0.1 ug/ml in ELISA Buffer and use it fresh.
  3. Reconstitute the Streptavidin-HRP (HRP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted HRP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
  4. Reconstitute the Standard Protein (STD) (recombinant human IL-36 gamma/IL-1F9) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml, 7.8 pg/ml, 3.9 pg/ml and 0 pg/ml.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for IL-36 gamma/IL-1F9 Antibody Pair

• IL-1 epsilon
• IL-1 H1
• IL-1 Related Protein 2
• IL-1(EPSILON)
• IL1E
• IL-1-epsilon
• IL1F9
• IL-1F9
• IL1H1
• IL-1H1
• IL-1-Related Protein 2
• IL1RP2
• IL-1rp2
• IL36 gamma
• IL-36 gamma
• IL36G
• interleukin 1 family, member 9
• interleukin 1-related protein 2
• Interleukin 36, Gamma
• Interleukin-1 epsilon
• interleukin-1 family member 9
• Interleukin-1 homolog 1
• Interleukin-36 Gamma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL36G