IL-36 gamma/IL-1F9 Antibody (278706) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 278706
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

IL-36 gamma/IL-1F9 Antibody (278706) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human IL‑36γ/IL‑1F9
  Met1-Asp169
  Accession # Q9NZH8
• Specificity: Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha , rhIL-1 beta , rhIL-36Ra/IL-1F5, rhIL-36 alpha /IL-1F6, rhIL-1F7, rhIL-36 beta /IL-1F8, rhIL-1ra/IL-1F3, or rhIL-18/IL-1F4 is
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 gamma/IL-1F9 Antibody (278706) [Alexa Fluor® 350]

• IL-1 epsilon
• IL-1 H1
• IL-1 Related Protein 2
• IL-1(EPSILON)
• IL1E
• IL-1-epsilon
• IL1F9
• IL-1F9
• IL1H1
• IL-1H1
• IL-1-Related Protein 2
• IL1RP2
• IL-1rp2
• IL36 gamma
• IL-36 gamma
• IL36G
• interleukin 1 family, member 9
• interleukin 1-related protein 2
• Interleukin 36, Gamma
• Interleukin-1 epsilon
• interleukin-1 family member 9
• Interleukin-1 homolog 1
• Interleukin-36 Gamma

Background

Interleukin-36 gamma (IL-36 gamma ) [also named IL-1F9, IL-1 epsilon  (epsilon), and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta , IL-1 alpha , IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 gamma is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293T cells (5). Human to mouse, IL-36 gamma has 53% aa sequence identity. Within the family, IL-36 gamma shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta , IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-36 gamma include Langerhans cells, keratinocytes/stratified squamous epithelium, plus chief cells and parietal cells of the stomach (4, 5). The receptor for IL-36 gamma is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-36 gamma , along with IL-36 beta /IL-1F8 and IL-36 alpha /IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.