Recombinant Mouse IL-28B/IFN-lambda 3 Protein

SDS-Page: Recombinant Mouse IL-28B/IFN-lambda 3 Protein [NBP2-35077] - Mouse IL-28B/IFN-lambda 3 Protein [NBP2-35077]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse IL-28B/IFN-lambda 3 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to IL-28B/IFN-lambda 3 Source: E. coli

  Uniprot ID: Q8CGK6

  Amino Acid Sequence: DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV

• Details of Functionality: IL-28B/IFN-lambda 3 protein is fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x 10^4 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IFNL3
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-28B/IFN-lambda 3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse IL-28B/IFN-lambda 3 Protein

• interleukin-28B
• IFN-lambda 3
• IFN-lambda 3/IL-28B
• IL28B
• IL-28B
• IL28C
• interferon, lambda 3

Background

Interferon lambda 3 (IFN-lambda3), also named as interleukin 28B (IL28B), is a protein encoded by the IL28B gene. IL28B, IL28A and IL29 are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by these three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor beta (IL-10Rbeta) and interleukin 28 receptor alpha (IL-28Ralpha). IFN-lambda3 is a cytokine with immune-modulatory activity. It up regulates MHC class I antigen expression and displays potent antiviral activity and antitumor activity, also plays an important role in Toll-like receptor (TLR)- induced antiviral defense and in the antiviral immune defense in the intestinal epithelium. In individuals with chronic hepatitis C, the presence of specific polymorphisms near this gene has been associated with an increased response in treatment for the condition with interferon and ribavirin. The same polymorphisms were also demonstrated at higher frequencies in individuals infected with hepatitis C who cleared the infection spontaneously, compared to those that developed chronic hepatitis from the condition. Murine IFN-lambda3 shares 63 % amino acid sequence identity with human IFN-lambda3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IFNL3
• Uniprot:
  • Q8CGK6()
  • Q8CGK6()
  • Q8CGK6()