IL-28A/B (IFN-lambda 2/3) Antibody (244716) [Unconjugated]

Recombinant Mouse IL‑28B/IFN‑ lambda 3 (Catalog # 1789‑ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IL‑28B/IFN‑ lambda 3 (400 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑28B/IFN‑ lambda 3 Monoclonal Antibody (Catalog # MAB17892). The ND50 is typically 3-9 µg/mL.

• Reactivity: Mu
• Applications: B/N, ELISA(Cap)
• Clone: 244716
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-28A/B (IFN-lambda 2/3) Antibody (244716) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑28B/IFN‑ lambda 3
  Asp20-Val193
  Accession # Q8CGK6
• Specificity: Detects mouse IL‑28B/IFN‑ lambda 3 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant mouse IL-28A/IFN-lambda 2 and no cross-reactivity with recombinant human (rh) IL‑28A or rhIL-29 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 3-9 ug/mL
• Application Notes: ELISA Capture: Mouse IL-28A/B (IFN-lambda 2/3) Antibody (Catalog # MAB17892)
  ELISA Detection: Mouse IL-28A/B (IFN-lambda 2/3) Biotinylated Antibody (Catalog # BAM17891)
  Standard: Recombinant Mouse IL-28B/IFN-lambda 3 (Catalog # 1789-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28A/B (IFN-lambda 2/3) Antibody (244716) [Unconjugated]

• IFNl2
• IFNlambda 2
• IL28A
• IL-28A
• IL-28A/B (IFN-lambda 2/3)
• IL28B
• interferon lambda 2

Background

Human IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11‑13% amino acid (aa) sequence identity) and the type I IFN family (15‑19% aa sequence identity) (1‑3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double‑stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up‑regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor  alpha (IL-28 R alpha , also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Mouse IL‑28B cDNA encodes a 193 amino acid residue precursor protein with a putative 15 aa signal peptide. It shares 61%, 62% and 52% aa sequence identity with human IL-28A, human IL-28B and human IL-29, respectively.

1. Vilcek, J. (2003) Nature Immunol. 4:8.
2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-28A/B (IFN-lambda 2/3) Antibody (MAB17892)(11)

Publications using MAB17892

• Gaur, KK;Asuru, TR;Srivastava, M;Singh, N;Purushotham, N;Poojary, B;Das, B;Bhattacharyya, S;Asthana, S;Guchhait, P; 7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively EMBO molecular medicine 2024-09-16 [PMID: 39284947] (Neutralization, Mouse)
• De, M;Sukla, S;Bharatiya, S;Keshri, S;Roy, DG;Roy, S;Dutta, D;Saha, S;Ejazi, SA;Ravichandiran, V;Ali, N;Chatterjee, M;Chinnaswamy, S; IFN-?3 is induced by Leishmania donovani and can inhibit parasite growth in cell line models but not in the mouse model, while it shows a significant association with leishmaniasis in humans Infection and immunity 2024-01-09 [PMID: 38193711] (In vivo assay, Mouse)
• Krishna Latha, Yesha Patel, Sanjana Rao, Wendy T. Watford The Influenza-Induced Pulmonary Inflammatory Exudate in Susceptible Tpl2-Deficient Mice Is Dictated by Type I IFN Signaling Inflammation 2/1/2023 [PMID: 36227523]
• Krishna Latha, Yesha Patel, Sanjana Rao, Wendy T. Watford The Influenza-Induced Pulmonary Inflammatory Exudate in Susceptible Tpl2-Deficient Mice Is Dictated by Type I IFN Signaling Inflammation 2023-02-01 [PMID: 36227523] (In vivo assay, Mouse)
• Alexis R. Gibson, Adam Sateriale, Jennifer E. Dumaine, Julie B. Engiles, Ryan D. Pardy, Jodi A. Gullicksrud et al. A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition PLOS Pathogens 5/18/2022 [PMID: 35584177]
• Alexis R. Gibson, Adam Sateriale, Jennifer E. Dumaine, Julie B. Engiles, Ryan D. Pardy, Jodi A. Gullicksrud et al. A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition PLOS Pathogens 2022-05-18 [PMID: 35584177] (In vivo assay, Mouse)
• Y Zhan, I Kong, M Chopin, C Macri, JG Zhang, J Xie, SL Nutt, M O'Keeffe, ED Hawkins, EF Morand, AM Lew Plasmacytoid dendritic cells from parent strains of the NZB/W F1 lupus mouse contribute different characteristics to autoimmune propensity Immunol. Cell Biol., 2020-02-05;0(0):. 2020-02-05 [PMID: 31916630] (ELISA Capture, Mouse)
• SH Ferguson, DM Foster, B Sherry, ST Magness, DM Nielsen, JL Gookin Interferon-?3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection Cell Mol Gastroenterol Hepatol, 2019-03-05;0(0):. 2019-03-05 [PMID: 30849550] (Neutralization, Mouse, Porcine)
• Achille Broggi, Yunhao Tan, Francesca Granucci, Ivan Zanoni IFN-lambda suppresses intestinal inflammation by non-translational regulation of neutrophil function Nature Immunology 10/1/2017 [PMID: 28846084]
• Crotta S, Davidson S, Mahlakoiv T, Desmet C, Buckwalter M, Albert M, Staeheli P, Wack A Type I and type III interferons drive redundant amplification loops to induce a transcriptional signature in influenza-infected airway epithelia. PLoS Pathog, 2013-11-21;9(11):e1003773. 2013-11-21 [PMID: 24278020] (Neutralization, Mouse)
• Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE). PLoS ONE, 2010-01-14;5(1):e8720. 2010-01-14 [PMID: 20090936] (ELISA Development, Mouse)

Bioinformatics

• Uniprot:
  • Q8CGK6()