IL-22R alpha 1 Antibody (496514) [PerCP (Peridinin-chlorophyll Protein Complex)]

Hepa 1-6 mouse hepatoma cell line was stained with Rat Anti-Mouse IL-22 Ra1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB42941C, filled histogram) or isotype control antibody (IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

C2C12 cells were stained with Rat Anti-Mouse IL‑22 R alpha 1 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB42941C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 496514
• Clonality: Monoclonal
• Host: Rat
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

IL-22R alpha 1 Antibody (496514) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1
  Thr18-Ala228
  Accession # Q80XZ4
• Specificity: Detects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL‑22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha , recombinant mouse (rm) IL-20 R alpha , rhIL-22BP, or rmIL-22BP is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL22RA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22R alpha 1 Antibody (496514) [PerCP (Peridinin-chlorophyll Protein Complex)]

• CRF2-9
• CRF2-9interleukin 22 receptor
• Cytokine receptor class-II member 9
• Cytokine receptor family 2 member 9
• IL-22 R alpha 1
• IL-22 receptor subunit alpha-1
• IL22R alpha 1
• IL22R
• IL22R1
• IL22RA1
• IL-22Ra1
• IL-22R-alpha-1
• IL-TIF-R1
• interleukin 22 receptor, alpha 1
• interleukin-22 receptor subunit alpha-1
• zcytoR11

Background

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). Mouse IL-22 R alpha 1 contains a 215 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic region (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%‑26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL‑22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6‑9). IL‑22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL‑22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11‑14). IL‑22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL‑22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

1. Tachiiri, A. et al. (2003) Genes Immun. 4:153.
2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
3. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335. 
4. Boniface, K. et al. (2005) J. Immunol. 174:3695. 
5. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. 
6. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. 
7. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. 
8. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
9. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
10. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
12. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
13. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
14. Wolk, K. et al. (2004) Immunity 21:241.
15. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IL-22R alpha 1 Antibody (FAB42941C) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer IF Rat 01/26/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID: 24527311
• Species: Rat

Bioinformatics

• Gene Symbol: IL22RA1
• Uniprot:
  • Q80XZ4()